Application
Immunohistochemistry of IL-16 in mouse brain tissue with IL-16 antibody at 2.5 μg/mL.Application
Immunofluorescence of IL-16 in mouse brain tissue with IL-16 antibody at 20 μg/mL.Application
Western blot analysis of IL-16 in rat brain tissue lysate with IL-16 antibody at (A) 1 μg/ml and (B) 2 μg/mL.| Product Name | IL-16 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL-16 Antibody: LCF, NIL16, PRIL16, prIL-16, Pro-interleukin-16, Lymphocyte chemoattractant factor, IL-16 |
| Product description | IL-16 Antibody: IL-16 was initially identified as a chemotactic cytokine, but is now known to possess a wide range of activities. Later studies have more fully characterized IL-16 as an immunomodulatory cytokine that contributes to the regulatory process of CD4+ T cell recruitment and activation at sites of inflammation in association with asthma and several autoimmune diseases. The precursor of IL-16 (pro-IL-16) is thought to be cleaved towards the C-terminal region by Caspase-3, releasing a 20 kDa active form that binds to and signals through CD4. Besides acting as a chemotactic cytokine, IL-16 is thought to also be involved in the regulation of T cell proliferation and multiple infectious, immune-mediated, and autoimmune inflammatory disorders including irritable bowel syndrome, systemic lupus erythematosus, and neurodegenerative disorders. At least two isoforms of IL-16 are known to exist; the longer isoform (also known as NIL-16) is detected only in neurons of the cerebellum and hippocampus.1) Cruikshank WW, Center DM, Nisar N, et al. Molecular and functional analysis of a lymphocyte chemoattractant factor: association of biologic function with CD expression. Proc. Natl. Acad. Sci. USA1994; 91:5109-13. |
| Immunogen | IL-16 antibody was raised against a 20 amino acid synthetic peptide near the amino terminus of human IL-16. The immunogen is located within the first 50 amino acids of IL-16. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| IL-16 antibody can be used for detection of IL-16 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL16 |
|---|---|
| Alternative Names | IL-16 Antibody: LCF NIL16 PRIL16 prIL-16 Pro-interleukin-16 Lymphocyte chemoattractant factor IL-16 |
| Tissue Specificity | This antibody will only detect the NIL-16 isoform. |
Application
Immunohistochemistry of IL-16 in mouse brain tissue with IL-16 antibody at 2.5 μg/mL.
Application
Immunofluorescence of IL-16 in mouse brain tissue with IL-16 antibody at 20 μg/mL.
Application
Western blot analysis of IL-16 in rat brain tissue lysate with IL-16 antibody at (A) 1 μg/ml and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | IL-16 antibody can be used for detection of IL-16 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IL-16 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IL-16 Antibody is supplied in PBS containing 0.02% sodium azide. |
