Application
To detect hIL-16 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hIL-16 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hIL-16 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hIL-16 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hIL-16 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IL-16 (XP-5170Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-16.| Product Name | IL-16 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LCF, NIL16, PRIL16, prIL-16 |
| Product description | IL16 is a proinflammatory cytokine that is chemotactic for CD4+ T lymphocytes, monocytes, and eosinophils. IL16 is synthesized as a precursor molecule (pro IL16) of approximately 68 kDa and 631 amino acid residues lacking a signal peptide. The sequence and structure of IL16 is conserved across species. In addition to inducing chemotaxis, IL16 upregulates the IL2 receptor and upregulates HLADR expression. It also inhibits T cell receptor (TCR)/CD3 dependent activation, and suppresses HIV1 replication in vitro. Sources of IL16 include epithelial cells, mast cells, T lymphocytes (CD4+ and CD8+), macrophages, synovial fibroblasts, and eosinophils. |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-16 (human Interleukin-16). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-16 (4.20 ng/mL), a concentration of 0.12 - 0.15 μg/mL of this antibody is required. ELISA: To detect hIL-16 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-16. Sandwich: To detect hIL-16 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-16 (XP-5170Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-16. Western Blot: To detect hIL-16 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-16 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL16 |
|---|---|
| Alternative Names | LCF NIL16 PRIL16 prIL-16 |
Application
To detect hIL-16 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hIL-16 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hIL-16 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hIL-16 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hIL-16 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IL-16 (XP-5170Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-16.| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-16 (4.20 ng/mL), a concentration of 0.12 - 0.15 μg/mL of this antibody is required. ELISA: To detect hIL-16 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-16. Sandwich: To detect hIL-16 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-16 (XP-5170Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-16. Western Blot: To detect hIL-16 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-16 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | IL-16 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
