Application
To detect mIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-17A is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect mIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-17A is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect mIL-17A by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine IL-17A (38-197) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-17A.| Product Name | IL-17A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Il17, Ctla8, IL-17, Ctla-8, IL-17A, Il17, Interleukin-17A, Cytotoxic T-lymphocyte-associated antigen 8 |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-17A (murine IL-17A). Murine IL-17A specific antibody was purified by affinity chromatography employing immobilized mIL-17A matrix. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| ELISA: Indirect: To detect mIL-17A by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-17A. Sandwich To detect mIL-17A by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine IL-17A as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-17A. Western Blot: To detect mIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-17A is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | Il17a |
|---|---|
| Alternative Names | Il17 Ctla8 IL-17 Ctla-8 IL-17A Il17 Interleukin-17A Cytotoxic T-lymphocyte-associated antigen 8 |
Application
To detect mIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-17A is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect mIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-17A is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect mIL-17A by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine IL-17A (38-197) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-17A.| Application Notes | ELISA: Indirect: To detect mIL-17A by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-17A. Sandwich To detect mIL-17A by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine IL-17A as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-17A. Western Blot: To detect mIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-17A is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | IL-17A antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
