Application
This antibody stained colchicine injected mouse brain (polymorph layer of the dentate gyrus) tissue. The primary antibody was incubated at 0.5 ug/ml overnight at 4˚C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.Application
To detect mIL-1-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect mIL-1-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect mIL-1-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine IL-1-beta (XP-5179Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-1-beta.| Product Name | IL-1B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Il-1b, IL-1betaInterleukin-1 betaIL-1 beta |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-1-beta (murine IL-1 beta). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mIL-1β (10 pg/mL), a concentration of 0.57-0.86 μg/mL of this antibody is required. ELISA: To detect mIL-1 beta by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-1 beta. To detect mIL-1β by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine IL-1β (XP-5179Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-1β. Western Blot: To detect mIL-1 beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1 beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Il1b |
|---|---|
| Alternative Names | Il-1b IL-1betaInterleukin-1 betaIL-1 beta |
Application
This antibody stained colchicine injected mouse brain (polymorph layer of the dentate gyrus) tissue. The primary antibody was incubated at 0.5 ug/ml overnight at 4˚C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.
Application
To detect mIL-1-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect mIL-1-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect mIL-1-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine IL-1-beta (XP-5179Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-1-beta.| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mIL-1β (10 pg/mL), a concentration of 0.57-0.86 μg/mL of this antibody is required. ELISA: To detect mIL-1 beta by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-1 beta. To detect mIL-1β by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine IL-1β (XP-5179Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-1β. Western Blot: To detect mIL-1 beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1 beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | IL-1B antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM281889