Welcome to Omnimabs!
TEL: 02154156726 Info: info@Omnimabs.com CN
IL1β Rabbit mAb
Catalog Number:OM644085
OM644085
Discount Price
OM644085-100ul
100ul
OM644085-50ul
50ul
OM644085-20ul
20ul
Product Profile
Product Name IL1β Rabbit mAb
Antibody Type Primary Antibodies
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 118-269 of mouse IL1β (NP_032387.1).
Key Feature
Clonality monoclonal
Isotype IgG
Host Species Rabbit
Tested Applications ICC/IFWB
WB:1:1000-1:5000
ICC/IF:1:100-1:400
Species Reactivity Mouse
Concentration 1mg/ml
Purification Affinity purified
Target Information
Gene Symbol Il1b
Gene Synonyms Il-1b
IL-1beta
Gene Full Name interleukin 1 beta
Gene Summary The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1. The encoded protein plays a role in thymocyte proliferation and is involved in the inflammatory response. [provided by RefSeq, Aug 2015]
Molecular Weight(MW) 19kDa/17kDa/31kDa
Cellular Localization cytosol, extracellular region, extracellular space, lysosome.
Application

WB

Western blot analysis of lysates from RAW 264.7 cells using IL1β Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. Raw264.7 cells were treated by LPS (1μg/ml) at 37℃ for 8 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

ICC/IF

Confocal imaging of RAW 264.7 cells (treated with BFA and LPS) and RAW 264.7 cells (untreated) using IL1β Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
Application Notes WB:1:1000-1:5000
ICC/IF:1:100-1:400
Additional Information
Form Liquid
Storage Instructions Store at -20℃. Avoid freeze / thaw cycles.
Storage Buffer Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
validation
question title
question detaiil
Verification code
 

References(0)