WB
Western blot analysis using Interleukin 1 beta antibody against NIH 3T3 (1), K562 (2) cell lysate.12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (
OM790003), and block it with TBST (
OM750016) plus skimmed milk powder for one hour. Dilute the primary antibody with the antibody diluent (
OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (
OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (
OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (
OM750016) again, 5 minutes each time. Use ECL (
OM625701) for luminescence.staining time: 60S.
IHC
Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using Interleukin 1 beta antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (
OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (
OM750003). Block the tissue with 10% non-immune goat serum(
OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (
OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(
OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.