WB
Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (NC) , human IL1B plasmid (hIL1B), mouse IL1B plasmid (mIL1B), rat IL1B plasmid (rIL1B) using anti-IL1B antibody(1:500).IHC
Immunohistochemical staining of paraffin embedded Adenocarcinoma of Human endometrium tissue using anti-IL1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)ICC/IF
Anti-IL1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6 ENTRY IL1B.| Product Name | IL1 beta (IL1B) Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Full length human recombinant protein of human IL1B(NP_000567) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:4000 IHC: 1:150 ICC: 1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL1B |
|---|---|
| Gene Synonyms | IL-1 IL1F2 IL1beta IL1-BETA |
| Gene Full Name | interleukin 1 beta |
| Gene Summary | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. Similarly, IL-1B has been implicated in human osteoarthritis pathogenesis. Patients with severe Coronavirus Disease 2019 (COVID-19) present elevated levels of pro-inflammatory cytokines such as IL-1B in bronchial alveolar lavage fluid samples. The lung damage induced by the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL-1B. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2020] |
| Molecular Weight(MW) | 31 kDa |
| Cellular Localization | Cytoplasm,Lysosome,Secreted. |
WB
Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (NC) , human IL1B plasmid (hIL1B), mouse IL1B plasmid (mIL1B), rat IL1B plasmid (rIL1B) using anti-IL1B antibody(1:500).
IHC
Immunohistochemical staining of paraffin embedded Adenocarcinoma of Human endometrium tissue using anti-IL1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
ICC/IF
Anti-IL1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6 ENTRY IL1B.| Application Notes | WB:1:1000-1:4000 IHC: 1:150 ICC: 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C as received.Stable for 12 months from date of receipt. |
| Storage Buffer | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
Data sheet for OM643615