Welcome to Omnimabs!
TEL: 02154156726 Info: info@Omnimabs.com CN
IL1 beta (IL1B) Mouse Monoclonal Antibody
Catalog Number:OM643615
OM643615
Discount Price
OM643615-100ul
2~3 Week
100ul
OM643615-50ul
50ul
2~3 Week
Product Profile
Product Name IL1 beta (IL1B) Mouse Monoclonal Antibody
Antibody Type Primary Antibodies
Immunogen Full length human recombinant protein of human IL1B(NP_000567) produced in HEK293T cell.
Key Feature
Clonality Monoclonal
Isotype IgG1
Host Species Mouse
Tested Applications ICC/IFIHCWB
WB:1:1000-1:4000
IHC: 1:150
ICC: 1:100
Species Reactivity Human
Concentration 1mg/ml
Purification Affinity purified
Target Information
Gene Symbol IL1B
Gene Synonyms IL-1
IL1F2
IL1beta
IL1-BETA
Gene Full Name interleukin 1 beta
Gene Summary The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. Similarly, IL-1B has been implicated in human osteoarthritis pathogenesis. Patients with severe Coronavirus Disease 2019 (COVID-19) present elevated levels of pro-inflammatory cytokines such as IL-1B in bronchial alveolar lavage fluid samples. The lung damage induced by the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL-1B. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2020]
Molecular Weight(MW) 31 kDa
Cellular Localization Cytoplasm,Lysosome,Secreted.
Application

WB

Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (NC) , human IL1B plasmid (hIL1B), mouse IL1B plasmid (mIL1B), rat IL1B plasmid (rIL1B) using anti-IL1B antibody(1:500).

IHC

Immunohistochemical staining of paraffin embedded Adenocarcinoma of Human endometrium tissue using anti-IL1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

ICC/IF

Anti-IL1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6 ENTRY IL1B.
Application Notes WB:1:1000-1:4000
IHC: 1:150
ICC: 1:100
Additional Information
Form Liquid
Storage Instructions Store at -20°C as received.Stable for 12 months from date of receipt.
Storage Buffer PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
validation
question title
question detaiil
Verification code
 

References(0)