Application
Immunohistochemistry of IL-1F10 in human spleen tissue with IL-1F10 antibody at 5 μg/mL.Application
Western blot analysis of IL-1F10 in human liver tissue lysate with IL-1F10 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | IL-1F10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL-1F10 Antibody: IL-38, FKSG75, IL1HY2, IL-1HY2, IL1-theta, FIL1-theta, FIL1T, IL38, UNQ6119/PRO20041, Interleukin-1 family member 10, Family of interleukin 1-theta, IL-1F10 |
| Product description | The cytokine IL-1 is responsible for initiating a variety of activities through the activation of transcription factors, NF-kappa B and AP-1, thereby promoting host response to injury or infection (1). IL-1F10 (Interleukin-1 family member 10), also known as FIL1T, IL1HY2 or FKSG75, is highly expressed in the skin, spleen and proliferating B-cells of the tonsil (2). IL-1F10 binds soluble IL-1 receptor type 1, and is one of nine Interleukin 1 families clustered on chromosome 2, where it is thought to participate in the regulation of adapted and innate immune responses (3). IL-1F10 and IL-1RN polymorphisms may play an important role in the susceptibility to developing rheumatoid arthritis (4).1) Towne JE, Garka KE, Renshaw BR, et al. Interleukin (IL)-1F6, IL-1F8, and IL-1F9 signal through IL-1Rrp2 and IL-1RAcP to activate the pathway leading to NF-kappaB and MAPKs. J. Biol. Chem. 2004; 279:13677-88. |
| Immunogen | IL-1F10 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human IL-1F10. The immunogen is located within the last 50 amino acids of IL-1F10. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| IL-1F10 antibody can be used for detection of IL-1F10 by Western blot at 1 - 2 μg/ml: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL1F10 |
|---|---|
| Alternative Names | IL-1F10 Antibody: IL-38 FKSG75 IL1HY2 IL-1HY2 IL1-theta FIL1-theta FIL1T IL38 UNQ6119/PRO20041 Interleukin-1 family member 10 Family of interleukin 1-theta IL-1F10 |
| Molecular Weight(MW) | Predicted: 17 kDa Observed: 20 kDa |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | IL-1F10 antibody is human, mouse and rat reactive. Two isoforms of IL-1F10 exist. |
Application
Immunohistochemistry of IL-1F10 in human spleen tissue with IL-1F10 antibody at 5 μg/mL.
Application
Western blot analysis of IL-1F10 in human liver tissue lysate with IL-1F10 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1304 - Human Liver Tissue Lysate |
|---|---|
| Application Notes | IL-1F10 antibody can be used for detection of IL-1F10 by Western blot at 1 - 2 μg/ml: |
| Form | Liquid |
|---|---|
| Storage Instructions | IL-1F10 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | IL-1F10 antibody is supplied in PBS containing 0.02% sodium azide. |
