Application
Immunocystochemistry of IL-1RAcP in HeLa cells with IL-1RAcP antibody at 2 μg/ml.Application
Immunofluorescence of IL-1RAcP in HeLa cells with IL-1RAcP antibody at 5 μg/mL.Application
Western blot analysis of IL-1RAcP in HeLa whole cell lysate with IL-1RAcP antibody at 1 μg/mL.| Product Name | IL-1RAcP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL-1RAcP Antibody: IL1R3, C3orf13, IL-1RAcP, IL1R3, Interleukin-1 receptor accessory protein, Interleukin-1 receptor 3, IL-1 receptor accessory protein |
| Product description | IL-1RAcP Antibody: The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1RAcP forms a complex with IL-1RI in response to IL-1 treatment. The IL-1 receptor-associated kinase (IRAK), which mediates activation of NF-κB inducing kinae (NIK) and of NF-κB, recruits to the IL-1R complex through IL-1RAcP. IL-1 activation of stress-activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP. Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues.1) Greenfeder SA, Nunes P, Kwee L, et al. Molecular cloning and characterization of a second subunit of the interleukin 1 receptor complex. J. Biol. Chem. 1995; 270:13757-65. |
| Immunogen | IL-1RAcP antibody was raised against a 16 amino acid peptide near the carboxy terminus of human IL-1RAcP. The immunogen is located within the last 50 amino acids of IL-1RAcP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| IL-1RAcP antibody can be used for detection of IL-1RAcP by Western blot at 1 μg/mL. Antibody can also be used for Immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL1RAP |
|---|---|
| Alternative Names | IL-1RAcP Antibody: IL1R3 C3orf13 IL-1RAcP IL1R3 Interleukin-1 receptor accessory protein Interleukin-1 receptor 3 IL-1 receptor accessory protein |
| Molecular Weight(MW) | Predicted: 63 kDa Observed: 66 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat: (100%) |
| Tissue Specificity | This polyclonal antibody has no cross activity to other members in the IL-1 receptor family. |
| Entrez Gene | 3556 |
|---|---|
| Protein Accession | NP_001161401 |
Application
Immunocystochemistry of IL-1RAcP in HeLa cells with IL-1RAcP antibody at 2 μg/ml.
Application
Immunofluorescence of IL-1RAcP in HeLa cells with IL-1RAcP antibody at 5 μg/mL.
Application
Western blot analysis of IL-1RAcP in HeLa whole cell lysate with IL-1RAcP antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | IL-1RAcP antibody can be used for detection of IL-1RAcP by Western blot at 1 μg/mL. Antibody can also be used for Immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IL-1RAcP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IL-1RAcP Antibody is supplied in PBS containing 0.02% sodium azide. |
