Application
To detect rat IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant rat IL-2 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.Application
To detect rat IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant rat IL-2 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.Application
To a sandwich ELISA (assuming 100µl/well), a concentration of 14.0-16.0 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant rat IL-2 when used with ProSci's biotinylated antigen affinity purified anti-rat IL-2 (38-205) as the detection antibody at a concentration of at least 2.0-4.0 ug/ml.| Product Name | IL-2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Interleukin 2Interleukin-2, T-cell growth factor, IL-2, TCGF, lymphokine |
| Immunogen | E.coli derived Recombinant Rat IL-2 |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: Sandwich: In a sandwich ELISA (assuming 100μL/well), a concentration of 14.0-16.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant rat IL-2 when used with ProSci’s Polyclonal biotinylated antigen affinity purified anti-rat IL-2 (38-205) as the detection antibody at a concentration of at least 2.0-4.0 μg/mL. Western Blot: To detect rat IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant rat IL-2 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.: |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL2 |
|---|---|
| Alternative Names | Interleukin 2Interleukin-2 T-cell growth factor IL-2 TCGF lymphokine |
Application
To detect rat IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant rat IL-2 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
Application
To detect rat IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant rat IL-2 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
Application
To a sandwich ELISA (assuming 100µl/well), a concentration of 14.0-16.0 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant rat IL-2 when used with ProSci's biotinylated antigen affinity purified anti-rat IL-2 (38-205) as the detection antibody at a concentration of at least 2.0-4.0 ug/ml.| Application Notes | ELISA: Sandwich: In a sandwich ELISA (assuming 100μL/well), a concentration of 14.0-16.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant rat IL-2 when used with ProSci’s Polyclonal biotinylated antigen affinity purified anti-rat IL-2 (38-205) as the detection antibody at a concentration of at least 2.0-4.0 μg/mL. Western Blot: To detect rat IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant rat IL-2 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
