Application
To detect mIL-21 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine IL-21 (38-213) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-21.| Product Name | IL-21 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Interleukin-21IL-21 |
| Product description | A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations. |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-21 (Murine Interleukin-21). Murine IL-21 specific antibody was purified by affinity chromatography employing immobilized mIL-21 matrix. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: Indirect: To detect mIL-21 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-21. Sandwich To detect mIL-21 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine IL-21 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-21. Western Blot: To detect mIL-21 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-21 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
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| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Gene Symbol | Il21 |
|---|---|
| Alternative Names | Interleukin-21IL-21 |
Application
To detect mIL-21 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine IL-21 (38-213) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-21.| Application Notes | ELISA: Indirect: To detect mIL-21 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-21. Sandwich To detect mIL-21 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine IL-21 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-21. Western Blot: To detect mIL-21 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-21 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | IL-21 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
