Application
Immunohistochemistry of IL-21 receptor in rat lung with IL-21 receptor antibody at 10 μg/mL.Application
Immunofluorescence of IL-21 Receptor in Rat Lung cells with IL-21 Receptor antibody at 20 μg/mL.Application
Western blot analysis of IL-21 receptor expression in human HepG2 cell lysate with IL-21 receptor antibody at (A) 1 and (B) 2 μg /ml.| Product Name | IL-21 Receptor Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | IL-21 Receptor Antibody: A novel cytokine related to IL-2 and IL-15 was recently identified and designated IL-21. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor gamma chain, gammac, and mediates IL-21 signaling. Both IL-21R and the gammac are necessary for the IL-21 function. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21R is expressed in spleen, thymus, natural killer (NK), T and B cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.1) Parrish-Novak J, Dillon SR, Nelson A, et al. Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function. Nature. 2000;408(6808):57-63. |
| Immunogen | IL-21 receptor antibody was raised against a synthetic peptide corresponding to amino acids 97 to 111 of human IL-21 receptor precursor. The immunogen is located within amino acids 80 - 130 of IL-21 Receptor. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA,IF,IHC-P,WB |
| IL-21 receptor antibody can be used for detection of IL-21 receptor by Western blot at 1-2 μg/mL. An approximately 60 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL21R |
|---|---|
| Alternative Names | IL-21 Receptor Antibody: NILR CD360 NILR UNQ3121/PRO10273 Interleukin-21 receptor Novel interleukin receptor IL-21 receptor |
| Molecular Weight(MW) | Predicted: 60 kDa Observed: 62 kDa |
| Sequence Similarities | 2) Cat. No. 1462 - Rat Lung Tissue Lysate |
Application
Immunohistochemistry of IL-21 receptor in rat lung with IL-21 receptor antibody at 10 μg/mL.
Application
Immunofluorescence of IL-21 Receptor in Rat Lung cells with IL-21 Receptor antibody at 20 μg/mL.
Application
Western blot analysis of IL-21 receptor expression in human HepG2 cell lysate with IL-21 receptor antibody at (A) 1 and (B) 2 μg /ml.| Positive Control | 1) Cat. No. 1207 - Raji Cell Lysate |
|---|---|
| Application Notes | IL-21 receptor antibody can be used for detection of IL-21 receptor by Western blot at 1-2 μg/mL. An approximately 60 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | IL-21 Receptor antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IL-21 Receptor Antibody is supplied in PBS containing 0.02% sodium azide. |
