Application
To detect mIL-22 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-22 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect mIL-22 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-22 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect mIL-22 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine IL-22 (38-150) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-22.| Product Name | IL-22 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL-22, Iltif, IL-22a, ILTIFa, Il22a, Iltifa, Interleukin-22, IL-10-related T-cell-derived-inducible factor, IL-22 |
| Product description | A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations. |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-22. Murine IL-22 specific antibody was purified by affinity chromatography employing immobilized mIL-22 matrix. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: Direct: To detect mIL-22 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-22. Sandwich To detect mIL-22 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine IL-22 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-22. Western Blot: To detect mIL-22 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-22 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Gene Symbol | Il22 |
|---|---|
| Alternative Names | IL-22 Iltif IL-22a ILTIFa Il22a Iltifa Interleukin-22 IL-10-related T-cell-derived-inducible factor IL-22 |
Application
To detect mIL-22 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-22 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect mIL-22 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-22 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect mIL-22 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine IL-22 (38-150) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-22.| Application Notes | ELISA: Direct: To detect mIL-22 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-22. Sandwich To detect mIL-22 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine IL-22 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-22. Western Blot: To detect mIL-22 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-22 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | IL-22 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
