Application
Immunohistochemistry of IL-32 in human spleen tissue with IL-32 antibody at 10 μg/mL.Application
Immunofluorescence of IL-32 in Human Spleen cells with IL-32 antibody at 20 μg/mL.Application
Western blot analysis of IL-32 in human spleen lysate with IL-32 antibody at (A) 5 and (B) 10 μg/mL shows two isoforms of IL-32.| Product Name | IL-32 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL-32 Antibody: NK4, TAIF, TAIFa, TAIFb, TAIFc, TAIFd, IL-32beta, IL-32alpha, IL-32delta, IL-32gamma, NK4, Interleukin-32, Natural killer cells protein 4, IL-32 |
| Product description | IL-32 Antibody: Interleukin-32 (IL-32) was initially identified as a transcript (NK4) that is selectively expressed in lymphocytes and NK cells and whose expression is increased following activation by IL-2. It was later re-isolated from an IL-18-treated lung carcinoma cell line and re-named IL-32. IL-32 is unusual in that it does not share sequence homology with known cytokine families and is highly expressed in immune tissues, existing in at least four differentially spliced isoforms. Because treatment of human monocytic and mouse macrophage cells with IL-32 induces several proinflammatory cytokines such as TNF-α, IL-8 and MIP-2, and because it is also induced in human peripheral lymphocyte cells after mitogen stimulation and in epithelial cells by IFNγ, it has been suggested that IL-32 may play a role in autoimmune and inflammatory diseases such as rheumatoid arthritis.1) Dahl CA, Schall RP, He HL, et al. Identification of a novel gene expressed in activated natural killer cells and T cells. J. Immunol. 1992; 148:597-603. |
| Immunogen | IL-32 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human IL-32. The immunogen is located within the last 50 amino acids of IL-32. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| IL-32 antibody can be used for the detection of IL-32 by Western blot at 5 - 10 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL32 |
|---|---|
| Alternative Names | IL-32 Antibody: NK4 TAIF TAIFa TAIFb TAIFc TAIFd IL-32beta IL-32alpha IL-32delta IL-32gamma NK4 Interleukin-32 Natural killer cells protein 4 IL-32 |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | This antibody detects all isoforms of IL-32. |
Application
Immunohistochemistry of IL-32 in human spleen tissue with IL-32 antibody at 10 μg/mL.
Application
Immunofluorescence of IL-32 in Human Spleen cells with IL-32 antibody at 20 μg/mL.
Application
Western blot analysis of IL-32 in human spleen lysate with IL-32 antibody at (A) 5 and (B) 10 μg/mL shows two isoforms of IL-32.| Positive Control | 1) Cat. No. 1306 - Human Spleen Tissue Lysate |
|---|---|
| Application Notes | IL-32 antibody can be used for the detection of IL-32 by Western blot at 5 - 10 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IL-32 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IL-32 Antibody is supplied in PBS containing 0.02% sodium azide. |
