Application
Western blot analysis of IL-32 in Jurkat cell lysate with IL-32 antibody at (A) 2.5 and (B) 5 μg/mL.| Product Name | IL-32 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL-32 Antibody: NK4, TAIF, TAIFa, TAIFb, TAIFc, TAIFd, IL-32beta, IL-32alpha, IL-32delta, IL-32gamma, NK4, Interleukin-32, Natural killer cells protein 4, IL-32 |
| Product description | IL-32 Antibody: Interleukin-32 (IL-32) was initially identified as a transcript (NK4) that is selectively expressed in lymphocytes and NK cells and whose expression is increased following activation by IL-2. It was later re-isolated from an IL-18-treated lung carcinoma cell line and re-named IL-32. IL-32 is unusual in that it does not share sequence homology with known cytokine families and is highly expressed in immune tissues, existing in at least four differentially spliced isoforms. Because treatment of human monocytic and mouse macrophage cells with IL-32 induces several proinflammatory cytokines such as TNF-α, IL-8 and MIP-2, and because it is also induced in human peripheral lymphocyte cells after mitogen stimulation and in epithelial cells by IFNγ, it has been suggested that IL-32 may play a role in autoimmune and inflammatory diseases such as rheumatoid arthritis.1) Dahl CA, Schall RP, He HL, et al. Identification of a novel gene expressed in activated natural killer cells and T cells. J. Immunol. 1992; 148:597-603. |
| Immunogen | IL-32 antibody was raised against a 16 amino acid synthetic peptide from near the center of human IL-32. The immunogen is located within amino acids 50 - 100 of IL-32. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| IL-32 antibody can be used for the detection of IL-32 by Western blot at 2.5 - 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL32 |
|---|---|
| Alternative Names | IL-32 Antibody: NK4 TAIF TAIFa TAIFb TAIFc TAIFd IL-32beta IL-32alpha IL-32delta IL-32gamma NK4 Interleukin-32 Natural killer cells protein 4 IL-32 |
| Tissue Specificity | This antibody detects the largest isoform of IL-32. |
Application
Western blot analysis of IL-32 in Jurkat cell lysate with IL-32 antibody at (A) 2.5 and (B) 5 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | IL-32 antibody can be used for the detection of IL-32 by Western blot at 2.5 - 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IL-32 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IL-32 Antibody is supplied in PBS containing 0.02% sodium azide. |
