Application
Western blot analysis of IL-33 in A-20 cell lysate with IL-33 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of IL-33 in Human Lymph Node tissue with IL-33 antibody at 20 μg/mL.Application
Immunohistochemistry of IL-33 in human lymph node tissue with IL-33 antibody at 5 μg/ml.| Product Name | IL-33 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL-33 Antibody: DVS27, IL1F11, NF-HEV, NFEHEV, C9orf26, NFHEV, Interleukin-33, Interleukin-1 family member 11, IL-33 |
| Product description | IL-33 Antibody: Interleukin-33 (IL-33) is a recently identified member of the IL-1 family of cytokines whose other members include IL-1α/beta, IL-1Ra and IL-18. Its receptor has been shown to be ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and IL-1R accessory protein (IL-1RAcP). Receptor binding of IL-33 activates NF-κB and MAP kinases and induces the expression of TH2-associated cytokines such as IL-4, IL-5 and IL-6. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties.1) Schmitz J, Owyang A, Oldham E, et al. IL-33, and interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines. Immunity 2005; 23:479-90. |
| Immunogen | IL-33 antibody was raised against a 19 amino acid synthetic peptide from near the center of human IL-33. The immunogen is located within amino acids 150 - 200 of IL-33. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCWB |
| WB:1:500~1:2000 IF:1:100~1:500 IHC:1:50~1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL33 |
|---|---|
| Alternative Names | IL-33 Antibody: DVS27 IL1F11 NF-HEV NFEHEV C9orf26 NFHEV Interleukin-33 Interleukin-1 family member 11 IL-33 |
| Molecular Weight(MW) | Observed: 35 kDa |
Application
Western blot analysis of IL-33 in A-20 cell lysate with IL-33 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of IL-33 in Human Lymph Node tissue with IL-33 antibody at 20 μg/mL.
Application
Immunohistochemistry of IL-33 in human lymph node tissue with IL-33 antibody at 5 μg/ml.| Positive Control | A20 Cell Lysate, Human Lymphoid Tissue Tissue Slide |
|---|---|
| Application Notes | WB:1:500~1:2000 IF:1:100~1:500 IHC:1:50~1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | IL-33 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IL-33 Antibody is supplied in PBS containing 0.02% sodium azide. |
