Application
Immunohistochemistry of IL-33 in human lymph node tissue with IL-33 antibody at 5 μg/mL.Application
Western blot analysis of IL-33 using 125ng of recombinant IL-33 with IL-33 antibody at 1 μg/mL.| Product Name | IL-33 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL-33 Antibody [12H6D12] : DVS27, IL1F11, NF-HEV, NFEHEV, C9orf26 |
| Product description | IL-33 Monoclonal Antibody: Interleukin-33 (IL-33) is a recently identified member of the IL-1 family of cytokines whose other members include IL-1αβ, IL-1Ra and IL-18. Its receptor has been shown to be ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and IL-1R accessory protein (IL-1RAcP). Receptor binding of IL-33 activates NF-κB and MAP kinases and induces the expression of TH2-associated cytokines such as IL-4, IL-5 and IL-6. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE.1) Schmitz J, Owyang A, Oldham E, et al. IL-33, and interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines. Immunity 2005; 23:479-90. |
| Immunogen | Mouse monoclonal IL-33 antibody was raised against the recombinant IL-33 protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| IL-33 antibody can be used for the detection of IL-33 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL33 |
|---|---|
| Alternative Names | IL-33 Antibody [12H6D12] : DVS27 IL1F11 NF-HEV NFEHEV C9orf26 |
| Sequence Similarities | 2) Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide |
Application
Immunohistochemistry of IL-33 in human lymph node tissue with IL-33 antibody at 5 μg/mL.
Application
Western blot analysis of IL-33 using 125ng of recombinant IL-33 with IL-33 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 95-106 - IL-33 Recombinant Protein |
|---|---|
| Application Notes | IL-33 antibody can be used for the detection of IL-33 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IL-33 monoclonal antibody can be stored at -20˚C, stable for one year. |
| Storage Buffer | IL-33 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide. |
