Application
Immunohistochemistry of IL-36B in human lung tissue with IL-36B antibody at 2.5 μg/mL.Application
Immunofluorescence of IL-36B in human lung tissue with IL-36B antibody at 20 μg/mL.Application
Western blot analysis of IL-36B in A549 cell lysate with IL-36B antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | IL-36B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL-36B Antibody: FIL1, FIL1H, IL1F8, IL1H2, IL-1F8, IL-1H2, IL1-ETA, FIL1-(ETA), FILI-(ETA), Interleukin-36 beta, FIL1 eta, IL-1 eta |
| Product description | IL-36B is is a member of the interleukin 1 cytokine family whose gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2 (1). IL-36B is thought to activate the NF-kappaB pathway through IL-1 receptor family members IL-1RL2 and IL-1RAcP (2). Like the related proteins IL-36A and IL-36G, IL-36B requires post-translational processing for full agonist activity, but the cleavage mechanism is currently unknown (3). The IL-36 cytokines have been suggested to amplify Th1 responses by enhancing proliferation and Th1 polarization of naive CD4+ T cells (4).1) Smith DE, Renshaw BR, Ketchem RR, et al. Four new members expand the interleukin-1 superfamily. J. Biol. Chem. 2000; 275:1169-75. |
| Immunogen | IL-36B antibody was raised against a 16 amino acid peptide near the carboxy terminus of human IL-36B. The immunogen is located within the last 50 amino acids of IL-36B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| IL-36B antibody can be used for detection of IL-36B by Western blot at 1 - 2 μg/ml.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL36B |
|---|---|
| Alternative Names | IL-36B Antibody: FIL1 FIL1H IL1F8 IL1H2 IL-1F8 IL-1H2 IL1-ETA FIL1-(ETA) FILI-(ETA) Interleukin-36 beta FIL1 eta IL-1 eta |
| Molecular Weight(MW) | Predicted: 18 kDa Observed: 17 kDa |
| Sequence Similarities | 2) Cat. No. 10-101 - Human Lung Tissue Slide |
| Tissue Specificity | IL-36B antibody is human specific. At least two isoforms of IL-36B are known to exist; this antibody will only detect the longer isoform. IL-36B antibody will not cross-react with IL-36A or IL-36G. |
Application
Immunohistochemistry of IL-36B in human lung tissue with IL-36B antibody at 2.5 μg/mL.
Application
Immunofluorescence of IL-36B in human lung tissue with IL-36B antibody at 20 μg/mL.
Application
Western blot analysis of IL-36B in A549 cell lysate with IL-36B antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1203 - A549 Cell Lysate |
|---|---|
| Application Notes | IL-36B antibody can be used for detection of IL-36B by Western blot at 1 - 2 μg/ml.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IL-36B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | IL-36B antibody is supplied in PBS containing 0.02% sodium azide. |
