Application
To detect Murine IL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine IL-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect Murine IL-5 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine IL-5 (38-283) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IL-5.| Product Name | IL-5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Il-5, Il-5, Interleukin-5, B-cell growth factor II, IL-5 |
| Immunogen | Produced from sera of rabbits immunized with highly pure recombinant Murine IL-5. Murine IL-5 specific antibody was purified by affinity chromatography employing an immobilized Murine IL-5 matrix. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: Sandwich: To detect Murine IL-5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine IL-5 (38-283) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IL-5. Western Blot: To detect Murine IL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Murine IL-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Gene Symbol | Il5 |
|---|---|
| Alternative Names | Il-5 Il-5 Interleukin-5 B-cell growth factor II IL-5 |
Application
To detect Murine IL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine IL-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect Murine IL-5 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine IL-5 (38-283) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IL-5.| Application Notes | ELISA: Sandwich: To detect Murine IL-5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine IL-5 (38-283) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IL-5. Western Blot: To detect Murine IL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Murine IL-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
