Application
Immunohistochemical analysis of paraffin-embedded M. small intestine section using IL18 Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.Application
Western blot analysis of lysate from Hela cell line, using IL18 Antibody at 1:1000.Application
Immunohistochemical analysis of paraffin-embedded R. small intestine section using IL18 Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.Application
Fluorescent image of U251 cells stained with IL18 Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).Application
Flow cytometric analysis of Hela cells using IL18 Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.| Product Name | IL18 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Interleukin-18, IL-18, Iboctadekin, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma, IL18, IGIF, IL1F4 |
| Product description | Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.1) Ushio S.,et al.J. Immunol. 156:4274-4279(1996). |
| Immunogen | This IL18 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 155-188 amino acids from the C-terminal region of human IL18. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For IHC-P starting dilution is: 1:25 For IF starting dilution is: 1:25 For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL18 |
|---|---|
| Alternative Names | Interleukin-18 IL-18 Iboctadekin Interferon gamma-inducing factor IFN-gamma-inducing factor Interleukin-1 gamma IL-1 gamma IL18 IGIF IL1F4 |
| Molecular Weight(MW) | 22 kDa |
Application
Immunohistochemical analysis of paraffin-embedded M. small intestine section using IL18 Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Application
Western blot analysis of lysate from Hela cell line, using IL18 Antibody at 1:1000.
Application
Immunohistochemical analysis of paraffin-embedded R. small intestine section using IL18 Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Application
Fluorescent image of U251 cells stained with IL18 Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Application
Flow cytometric analysis of Hela cells using IL18 Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.| Application Notes | For IHC-P starting dilution is: 1:25 For IF starting dilution is: 1:25 For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
