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Monoclonal Antibody to Interleukin 18 (IL18)

Catalog Number:OM646524
OM646524
Discount Price
OM646524-100ul
100ul
Product Profile
Product Name Monoclonal Antibody to Interleukin 18 (IL18)
Antibody Type Primary Antibodies
Immunogen Recombinant IL18 (Tyr37~Asp193) expressed in E.coli.
Key Feature
Clonality Monoclonal
Isotype IgG2a,k
Host Species Mouse
Tested Applications IHCWB
WB:0.01-2µg/mL
IHC:5-40µg/mL
Species Reactivity Human
Concentration 1mg/ml
Purification Protein A/G
Target Information
Gene Symbol IL18
Gene Synonyms IGIF
IL-18
IL-1g
IL1F4
Gene Full Name interleukin 18
Gene Summary The protein encoded by this gene is a proinflammatory cytokine of the IL-1 family that is constitutively found as a precursor within the cytoplasm of a variety of cells including macrophages and keratinocytes. The inactive IL-18 precursor is processed to its active form by caspase-1, and is capable of stimulating interferon gamma production, and of regulating both T helper (Th) 1 and Th2 responses. This cytokine has been implicated in the injury of different organs, and in potentially fatal conditions characterized by a cytokine storm. In humans, IL-18 gene is located on chromosome 11. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2020]
Alternative Names IGIF
IL-1g
IL1F4
IL1-F4
Interferon-Gamma-Inducing Factor
Interleukin-1 Family Member 4
Iboctadekin
Interleukin-1 Gamma
Application

WB

Western Blot; Samples: Lane1: A431 cell lysate; Lane2: Human Leukocyte lysate; Primary Ab: 0.3µg/ml Mouse Anti-Human IL18 Antibody Second Ab: 0.2µg/ml HRP-Linked Caprine Anti Mouse IgG Polyclonal Antibody

IHC

DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 40µg/ml Mouse Anti-Human IL18 Antibody Second Ab: 2µg/mL HRP Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Application Notes WB:0.01-2µg/mL
IHC:5-40µg/mL
Additional Information
Form Liquid
Storage Instructions Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 24 months.
Storage Buffer Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300,50% glycerol.
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