Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
IL1RN Antibody (RB18901) IHC analysis in formalin fixed and paraffin embedded human Colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Confocal immunofluorescent analysis of IL1RN Antibody with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis of IL1RN using rabbit polyclonal IL1RN Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the IL1RN gene (Lane 2).| Product Name | IL1RN Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, ICIL-1RA, IL1 inhibitor, Anakinra, IL1RN, IL1F3, IL1RA |
| Product description | The protein is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. A polymorphism of its gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer.1) Kumar,S., Biochem. Biophys. Res. Commun. 387 (3), 456-460 (2009) |
| Immunogen | This IL1RN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-129 amino acids from the Central region of human IL1RN. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | IL1RN |
|---|---|
| Alternative Names | Interleukin-1 receptor antagonist protein IL-1RN IL-1ra IRAP ICIL-1RA IL1 inhibitor Anakinra IL1RN IL1F3 IL1RA |
| Molecular Weight(MW) | 20 kDa |
Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
IL1RN Antibody (RB18901) IHC analysis in formalin fixed and paraffin embedded human Colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Confocal immunofluorescent analysis of IL1RN Antibody with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis of IL1RN using rabbit polyclonal IL1RN Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the IL1RN gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
