Application
IL25 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western Blot at 1:1000 dilution Lane 1: human testis lysate Lane 2: PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).| Product Name | IL25 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Interleukin-25, IL-25, Interleukin-17E, IL-17E, IL25, IL17E |
| Product description | The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants.1) Xu, G., et al. Allergy 65(5):581-589(2010) |
| Immunogen | This IL25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-104 amino acids from the Central region of human IL25. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL25 |
|---|---|
| Alternative Names | Interleukin-25 IL-25 Interleukin-17E IL-17E IL25 IL17E |
| Molecular Weight(MW) | 20 kDa |
Application
IL25 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western Blot at 1:1000 dilution Lane 1: human testis lysate Lane 2: PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
