WB
Protein: spleen(mouse) lysate at 30ug; Primary: rabbit Anti-IL17E at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1: 5000; Predicted band size: 17 kD Observed band size: 17 kDIHC
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-IL17E Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining| Product Name | Anti-IL17E antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human IL17E: 431-434/644 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCWB |
| WB:1:500-2000 IHC-P:1:100-500 IHC-F:1:100-500 ICC:1:100-500 IF:1:100-500 ELISA:1:5000-10000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | IL25 |
|---|---|
| Gene Synonyms | Eukaryota Metazoa Chordata Craniata Vertebrata Euteleostomi Mammalia Eutheria Euarchontoglires Primates Haplorrhini Catarrhini Hominidae Homo |
| Gene Summary | The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010] |
| Molecular Weight(MW) | 17kDa |
| Cellular Localization | Secretory protein |
| SwissProt ID | Q9H293 |
|---|
WB
Protein: spleen(mouse) lysate at 30ug; Primary: rabbit Anti-IL17E at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1: 5000; Predicted band size: 17 kD Observed band size: 17 kD
IHC
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-IL17E Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining| Application Notes | WB:1:500-2000 IHC-P:1:100-500 IHC-F:1:100-500 ICC:1:100-500 IF:1:100-500 ELISA:1:5000-10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
