Application
Western blot analysis of IL2RG Antibody in HL-60 cell line lysates (35ug/lane)Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of lysate from Jurkat cell line, using IL2RG Antibody at 1:1000.Application
Western Blot at 1:2000 dilution Lane 1: human spleen lysates Lane 2: Jurkat whole cell lysates Lane 3: Daudi whole cell lysates Lane 4: KG-1 whole cell lysates Lane 5: MOLT-4 whole cell lysates Lane 6: HL-60 whole cell lysates Lysates/proteins at 20 ug per lane.Application
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized human spleen tissue labeling Pdx1 with AP9450a at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing membrane staining on human spleen tissue. The nuclear counter stain is DAPI (blue).| Product Name | IL2RG Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cytokine receptor common subunit gamma, Interleukin-2 receptor subunit gamma, IL-2 receptor subunit gamma, IL-2R subunit gamma, IL-2RG, gammaC, p64, CD132, IL2RG |
| Product description | IL2RG is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in this gene cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.1) Ross, J.A., et al. J. Biol. Chem. 285(6):3582-3591(2010) |
| Immunogen | This IL2RG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-101 amino acids from the N-terminal region of human IL2RG. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFWB |
| For IF starting dilution is: 1:25 For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL2RG |
|---|---|
| Alternative Names | Cytokine receptor common subunit gamma Interleukin-2 receptor subunit gamma IL-2 receptor subunit gamma IL-2R subunit gamma IL-2RG gammaC p64 CD132 IL2RG |
| Molecular Weight(MW) | 42 kDa |
Application
Western blot analysis of IL2RG Antibody in HL-60 cell line lysates (35ug/lane)
Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of lysate from Jurkat cell line, using IL2RG Antibody at 1:1000.
Application
Western Blot at 1:2000 dilution Lane 1: human spleen lysates Lane 2: Jurkat whole cell lysates Lane 3: Daudi whole cell lysates Lane 4: KG-1 whole cell lysates Lane 5: MOLT-4 whole cell lysates Lane 6: HL-60 whole cell lysates Lysates/proteins at 20 ug per lane.
Application
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized human spleen tissue labeling Pdx1 with AP9450a at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing membrane staining on human spleen tissue. The nuclear counter stain is DAPI (blue).| Application Notes | For IF starting dilution is: 1:25 For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
