Application
Immunocytochemistry of ILP-2 in HepG2 cells with ILP-2 antibody at 10 μg/mL.Application
Immunofluorescence of ILP-2 in HepG2 cells with ILP-2 antibody at 20 μg/mL.Application
Western blot analysis of ILP-2 expression in human HepG2 (lane 1) and MOLT4 (lane 2) cell lysates with ILP-2 antibody at 1 μg/mL.| Product Name | ILP-2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ILP-2 Antibody: ILP2, ILP-2, hILP2, ILP2, Baculoviral IAP repeat-containing protein 8, Inhibitor of apoptosis-like protein 2, IAP-like protein 2 |
| Product description | ILP-2 Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family including IAP, XIAP/ILP-1/MIHA, and Livin/KIAP that bind to and inhibits specific proteases. A novel member in the IAP protein family was recently identified and designated ILP-2 for IAP-like protein-2. ILP-2 has high homology to ILP-1, but is encoded by a distinct gene that is solely expressed in testis of tested normal human tissues. ILP-2, unlike ILP-1, has no inhibitory effect on Fas and TNF induced apoptosis, but potently inhibits apoptosis induced by overexpression of Bax or by coexpression of caspase-9 with Apaf-1. ILP-2 interacts with the processed caspase-9. These results suggest that ILP-2 is a novel IAP family member with restricted specificity for caspase-9.1) Richter BW, Mir SS, Eiben LJ, Lewis J, Reffey SB, Frattini A, Tian L, Frank S, Youle RJ, Nelson DL, Notarangelo LD, Vezzoni P, Fearnhead HO, Duckett CS. Molecular cloning of ILP-2, a novel member of the inhibitor of apoptosis protein family. Mol Cell Biol. 2001;21(13):4292-301. |
| Immunogen | ILP-2 antibody was raised with against a synthetic peptide corresponding to amino acids near the amino terminus of human ILP-2. The immunogen is located within the first 50 amino acids of ILP-2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| ILP-2 antibody can be used for detection of ILP-2 by Western blot at 1 μg/mL. A band at approximately 33 kDa can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BIRC8 |
|---|---|
| Alternative Names | ILP-2 Antibody: ILP2 ILP-2 hILP2 ILP2 Baculoviral IAP repeat-containing protein 8 Inhibitor of apoptosis-like protein 2 IAP-like protein 2 |
| Molecular Weight(MW) | 33 kDa |
| Sequence Similarities | 2) Cat. No. 17-011 - Hep G2 Cell Slide |
Application
Immunocytochemistry of ILP-2 in HepG2 cells with ILP-2 antibody at 10 μg/mL.
Application
Immunofluorescence of ILP-2 in HepG2 cells with ILP-2 antibody at 20 μg/mL.
Application
Western blot analysis of ILP-2 expression in human HepG2 (lane 1) and MOLT4 (lane 2) cell lysates with ILP-2 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | ILP-2 antibody can be used for detection of ILP-2 by Western blot at 1 μg/mL. A band at approximately 33 kDa can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ILP-2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ILP-2 Antibody is supplied in PBS containing 0.02% sodium azide. |
