Application
IMMT Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of hela cells using IMMT Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of IMMT using rabbit polyclonal IMMT Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the IMMT gene (Lane 2).Application
Western blot analysis of IMMT Antibody in Hela cell line lysates (35ug/lane)| Product Name | IMMT Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MICOS complex subunit MIC60, Cell proliferation-inducing gene 4/52 protein, Mitochondrial inner membrane protein, Mitofilin, p87/89, IMMT, HMP, MIC60, MINOS2 |
| Product description | Mitochondria are the center of cellular energy production and essential metabolic reactions. As double membrane-bound organelles, mitochondria from different species, tissues, and metabolic states are highly polymorphic in nature, yet exhibit common structural features. The ultrastructural variations in mitochondrial architecture occur mainly due to the differences in the amount and shape of cristae. Abundant cristae are found in mitochondria from tissues where energy demand is high. Analysis of the human heart mitochondrial proteome shows that mitofilin is one of the most abundant mitochondrial proteins. It appears to play an important role in the maintenance of cristae morphology.1) Bernert,G., et.al., Proteomics(12), 1752-1757 (2002) |
| Immunogen | This IMMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 700-729 amino acids from the C-terminal region of human IMMT. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | IMMT |
|---|---|
| Alternative Names | MICOS complex subunit MIC60 Cell proliferation-inducing gene 4/52 protein Mitochondrial inner membrane protein Mitofilin p87/89 IMMT HMP MIC60 MINOS2 |
| Molecular Weight(MW) | 84 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
IMMT Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of hela cells using IMMT Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of IMMT using rabbit polyclonal IMMT Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the IMMT gene (Lane 2).
Application
Western blot analysis of IMMT Antibody in Hela cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
