Application
Immunocytochemistry of INPP4B in 3T3 cells with INPP4B antibody at 2.5 μg/ml.Application
Western blot analysis of INPP4B in 3T3 cell lysate with INPP4B antibody at 1 μg/ml.| Product Name | INPP4B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Inositol polyphosphate-4-phosphatase type II 105kDa |
| Product description | Inositol polyphosphate 4-phosphatase type II (INPP4B) is one of the enzymes involved in phosphatidylinositol signaling pathways. It removes the phosphate group at position 4 of the inositol ring from inositol 3,4-bisphosphate (1). INPP4B can function as a tumor suppressor by inhibiting PI3K signaling. In human epithelial cells, knockdown of INPP4B expression resulted in enhanced Akt activation, anchorage-independent growth, and enhanced overall motility (2). It is thought INPP4B may also have oncogenic functions as INPP4B-dependent PI3K signaling is mediated by SGK3 in breast cancer cells and expression of INPP4B leads to enhanced SGK3 activation and suppression of AKT phosphorylation (3).1) Norris FA, Auethavekiat V, and Majerus PW. The isolation and characterization of cDNA encoding human and rat brain inositol polyphosphate 4-phosphase. J. Biol. Chem. 1995; 270:16128-33. |
| Immunogen | INPP4B antibody was raised against a 17 amino acid peptide near the amino terminus of human INPP4B. The immunogen is located within the last 50 amino acids of INPP4B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| INPP4B antibody can be used for detection of INPP4B by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | INPP4B |
|---|---|
| Alternative Names | Inositol polyphosphate-4-phosphatase type II 105kDa |
| Molecular Weight(MW) | Predicted: 102 kDa Observed: 100 kDa |
| Sequence Similarities | 2) Cat. No. 17-201 - 3T3/BALB Cell Slide |
| Tissue Specificity | INPP4B antibody is human, mouse and rat reactive. At least two isoforms of INPP4B are known to exist; this antibody only detects the longer isoform. INPP4B antibody is predicted to not cross-react with INPP4A. |
Application
Immunocytochemistry of INPP4B in 3T3 cells with INPP4B antibody at 2.5 μg/ml.
Application
Western blot analysis of INPP4B in 3T3 cell lysate with INPP4B antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | INPP4B antibody can be used for detection of INPP4B by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | INPP4B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | INPP4B antibody is supplied in PBS containing 0.02% sodium azide. |
