Application
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
INPP5B antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in CEM cell line lysates (35ug/lane).| Product Name | INPP5B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Type II inositol 1,5-trisphosphate 5-phosphatase, 75 kDa inositol polyphosphate-5-phosphatase, Phosphoinositide 5-phosphatase, 5PTase, INPP5B, OCRL2 |
| Product description | Cellular calcium signaling is controlled by the production of inositol phosphates (IPs) by phospholipase C in response to extracellular signals. The IP signaling molecules are inactivated by a family of inositol polyphosphate-5-phosphatases (5-phosphatases). INPP5B encodes the type II 5-phosphatase. The protein is localized to the cytosol and mitochondria, and associates with membranes through an isoprenyl modification near the C-terminus. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq].1) Coon, B.G., et al. Hum. Mol. Genet. 18(23):4478-4491(2009) |
| Immunogen | This INPP5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 950-979 amino acids from the C-terminal region of human INPP5B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | INPP5B |
|---|---|
| Alternative Names | Type II inositol 1 5-trisphosphate 5-phosphatase 75 kDa inositol polyphosphate-5-phosphatase Phosphoinositide 5-phosphatase 5PTase INPP5B OCRL2 |
| Molecular Weight(MW) | 113 kDa |
Application
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
INPP5B antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in CEM cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
