Application
Antibody used in WB on Human 721_B at 1 ug/ml.Application
Antibody used in WB on Human Muscle at 0.2-1 ug/ml.| Product Name | INSIG1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | INSIG1, MGC26273, |
| Product description | Oxysterols regulate cholesterol homeostasis through the liver X receptor (LXR)- and sterol regulatory element-binding protein (SREBP)-mediated signaling pathways. This gene is an insulin-induced gene. INSIG1 is an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. INSIG1 binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins.The protein encoded by this gene is highly similar to the protein product encoded by gene INSIG1. Both INSIG1 protein and this protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element binding proteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPs to the Golgi. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Li, C.G., (2008) Int. J. Cancer 123 (2), 273-282. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human INSIG1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| INSIG1 antibody can be used for detection of INSIG1 by ELISA at 1:1562500. INSIG1 antibody can be used for detection of INSIG1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | INSIG2 |
|---|---|
| Alternative Names | INSIG1 MGC26273 |
| Molecular Weight(MW) | 25 kDa |
Application
Antibody used in WB on Human 721_B at 1 ug/ml.
Application
Antibody used in WB on Human Muscle at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. XBL-10413 - Fetal Skeletal Muscle Tissue Lysate |
|---|---|
| Application Notes | INSIG1 antibody can be used for detection of INSIG1 by ELISA at 1:1562500. INSIG1 antibody can be used for detection of INSIG1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store INSIG1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
