Application
Western Blot at 1:2000 dilution + mouse brain lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: PC-12 whole cell lysate Lane 5: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis of anti-INSRR Pab in mouse brain lysateApplication
Formalin-fixed and paraffin-embedded human testis tissue reacted with INSRR antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | Insulin Receptor R Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Insulin receptor-related protein, IRR, IR-related receptor, Insulin receptor-related protein alpha chain, Insulin receptor-related protein beta chain, INSRR, IRR |
| Product description | Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).1) Shier, P., et al., J. Biol. Chem. 264(25):14605-14608 (1989). |
| Immunogen | This Insulin Receptor R antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-47 amino acids from the N-terminal region of human Insulin Receptor R. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | INSRR |
|---|---|
| Alternative Names | Insulin receptor-related protein IRR IR-related receptor Insulin receptor-related protein alpha chain Insulin receptor-related protein beta chain INSRR IRR |
| Molecular Weight(MW) | 144 kDa |
Application
Western Blot at 1:2000 dilution + mouse brain lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: PC-12 whole cell lysate Lane 5: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis of anti-INSRR Pab in mouse brain lysate
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with INSRR antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
