Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Integrin beta1 (Phospho-Thr789) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from HepG2 cells treated with Ca2+ using Integrin beta1 (Phospho-Thr789) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | Integrin beta1 (phospho Thr789) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD29, FNRB, ITB1 |
| Product description | Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and GNB2L1/RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis1) Argraves W.S., J. Cell Biol. 105:1183-1190(1987). |
| Immunogen | Integrin beta1 (phospho Thr789) antibody was raised against a peptide sequence around phosphorylation site of threonine 789 (V-E-T (p)-V-V) derived from Human Integrin beta1 . |
| Modification | p-Thr789 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ITGB1 |
|---|---|
| Alternative Names | CD29 FNRB ITB1 |
| Molecular Weight(MW) | 90 kDa |
| Tissue Specificity | The antibody detects endogenous levels of Integrin beta1 only when phosphorylated at threonine 789. |
| Entrez Gene | 3688 |
|---|---|
| Protein Accession | NP_002202.2 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Integrin beta1 (Phospho-Thr789) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from HepG2 cells treated with Ca2+ using Integrin beta1 (Phospho-Thr789) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
