Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Integrin β3 (Ab-773).Application
Western blot analysis of lysed extracts from HL60 and HT29 cells using Integrin β3 (Ab-773).| Product Name | Integrin beta3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GT, CD61, GP3A, BDPLT2, GPIIIa, BDPLT16, CREB2, CREBP1, Platelet membrane glycoprotein IIIa |
| Product description | Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.1) Chandhoke SK, et al. (2004) J Cell Sci; 117 (Pt 8): 1431-41. Datta A, et al. (2002) J Biol Chem; 277 (6): 3943-9. Law DA, et al. (1996) J Biol Chem; 271 (18): 10811-5. |
| Immunogen | Integrin beta3 (Ab-773) antibody was raised against a peptide sequence around aa. 771~775 (P-L-Y-K-E) derived from Human Integrin β3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ITGB3 |
|---|---|
| Alternative Names | GT CD61 GP3A BDPLT2 GPIIIa BDPLT16 CREB2 CREBP1 Platelet membrane glycoprotein IIIa |
| Molecular Weight(MW) | 110 kDa |
| Tissue Specificity | This antibody detects endogenous level of total Integrin β3 protein. |
| Entrez Gene | 3690 |
|---|---|
| Protein Accession | NP_000203.2 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Integrin β3 (Ab-773).
Application
Western blot analysis of lysed extracts from HL60 and HT29 cells using Integrin β3 (Ab-773).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
