Application
Immunocytochemistry of IQSEC1 in A-20 cells with IQSEC1 antibody at 2 μg/mL.Application
Immunofluorescence of IQSEC1 in A-20 cells with IQSEC1 antibody at 2 μg/mL.Application
Western blot analysis of IQSEC1 in A20 cell lysate with IQSEC1 antibody at 1 μg/ml.| Product Name | IQSEC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ADP ribosylation factors (Arfs) are small GTP-binding proteins known for their role in vesicular transport, where they nucleate the assembly of coat protein complexes at sites of carrier vesicle formation. IQSEC1 (GEP100/BRAG2), a guanine nucleotide exchange protein for ARF6, belongs to the BRAG family and contains one IQ domain, one PH domain and one SEC7 domain (1). As a known activator of ARF6, IQSEC1 regulates cell surface levels of Beta1 integrin and its knockdown blocked tumour metastasis (2,3). IQSEC1 links EGFR signaling to ARF6 activation and may contribute to the cancer metastasis and malignancy (4).1) Someya A, Sata M, Takeda K, et al. ARF-GEP(100), a guanine nucleotide-exchange protein for ADP-ribosylation factor 6. Proc. Natl. Acad. Sci. U.S.A. 2001; 98:2413-8. |
| Immunogen | IQSEC1 antibody was raised against a 19 amino acid peptide near the amino terminus of human IQSEC1. The immunogen is located within amino acids 60 - 110 of IQSEC1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| IQSEC1 antibody can be used for detection of IQSEC1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry at 2 μg/mL. For immunofluorescence start at 2 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IQSEC1 |
|---|---|
| Alternative Names | IQ motif and Sec7 domain 1 ADP-ribosylation factors guanine nucleotide-exchange protein 100 ARFGEP100 BRAG2 Brefeldin-resistant Arf-GEF 2 protein |
| Molecular Weight(MW) | Predicted: 106 kDa Observed: 103 kDa |
| Sequence Similarities | 2) Cat. No. 17-208 - A-20 Cell Slide |
| Tissue Specificity | IQSEC1 antibody is human, mouse and rat reactive. At least four isoforms of IQSEC1 are known to exist. IQSEC1 antibody is predicted to not cross-react with IQSEC2. |
Application
Immunocytochemistry of IQSEC1 in A-20 cells with IQSEC1 antibody at 2 μg/mL.
Application
Immunofluorescence of IQSEC1 in A-20 cells with IQSEC1 antibody at 2 μg/mL.
Application
Western blot analysis of IQSEC1 in A20 cell lysate with IQSEC1 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | IQSEC1 antibody can be used for detection of IQSEC1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry at 2 μg/mL. For immunofluorescence start at 2 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | IQSEC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | IQSEC1 antibody is supplied in PBS containing 0.02% sodium azide. |
