Application
Immunohistochemistry of IQSEC2 in mouse brain tissue with IQSEC2 antibody at 5 μg/ml.Application
Western blot analysis of IQSEC2 in SK-N-SH cell lysate with IQSEC2 antibody at 1 μg/ml.| Product Name | IQSEC2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ADP ribosylation factors (Arfs) are small GTP-binding proteins known for their role in vesicular transport, where they nucleate the assembly of coat protein complexes at sites of carrier vesicle formation. Similar to the related protein IQSEC1, IQSEC2 is a guanine nucleotide exchange protein for ARF1 and ARF6 that belongs to the BRAG family and contains one IQ domain, one PH domain and one SEC7 domain (1,2). IQSEC2 is a major component of the postsynaptic density (PSD) and colocalizes with PSD-95. It has been suggested to modulate the functions of the ARF proteins (1). Mutations in this gene have been implicated in nonsyndromic X-linked mental retardation (3).1) Murphy JA, Jensen ON, and Walikonis RS. BRAG1, a SEC7 domain-containing protein, is a component of the postsynaptic density of excitatory synapses. Brain Res. 2006; 1120:35-45. |
| Immunogen | IQSEC2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human IQSEC2. The immunogen is located within amino acids 1380 - 1430 of IQSEC2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| IQSEC2 antibody can be used for detection of IQSEC2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IQSEC2 |
|---|---|
| Alternative Names | IQ motif and Sec7 domain 2 Brefeldin-resistant Arf-GEF 1 protein BRAG1 MRX1 |
| Molecular Weight(MW) | Predicted: 104, 133, 141, 164 kDa Observed: 140 kDa |
| Tissue Specificity | IQSEC2 antibody is human, mouse and rat reactive. At least two isoforms of IQSEC2 are known to exist; this antibody will only detect the larger isoform. IQSEC2 antibody is predicted to not cross-react with IQSEC1. |
| Entrez Gene | 23096 |
|---|---|
| Protein Accession | NP_001104595 |
Application
Immunohistochemistry of IQSEC2 in mouse brain tissue with IQSEC2 antibody at 5 μg/ml.
Application
Western blot analysis of IQSEC2 in SK-N-SH cell lysate with IQSEC2 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | IQSEC2 antibody can be used for detection of IQSEC2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | IQSEC2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | IQSEC2 antibody is supplied in PBS containing 0.02% sodium azide. |
