Application
Western blot analysis of extracts from 293 cells treated with Heatshock using Phospho-IR (Tyr1355) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | IR (phospho Tyr1355) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Receptor tyrosine kinase which mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates. Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src-homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosines residues, including the p85 regulatory subunit of PI3K and SHP2.1) Tennagels N, et al. (2001) Biochem Biophys Res Commun 282, 387-93 |
| Immunogen | IR (phospho Tyr1355) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 1355 (R-S-Y (p)-E-E) derived from Human IR. |
| Modification | p-Tyr1355 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | INSR |
|---|---|
| Alternative Names | CD220 antigen IR insulin receptor kinase InsR |
| Molecular Weight(MW) | 95 kDa |
| Tissue Specificity | The antibody detects endogenous level of IR only when phosphorylated at tyrosine 1355. |
| Entrez Gene | 3643 |
|---|---|
| Protein Accession | NP_000199.2 |
Application
Western blot analysis of extracts from 293 cells treated with Heatshock using Phospho-IR (Tyr1355) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
