Application
Antibody used in WB on Human Liver at 0.2-1 ug/ml.Application
Antibody used in WB on Mouse Liver at: 1:50.| Product Name | IREB2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | IREB2 binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. IREB2 binds to the IRE element in ferritin which results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.1) Zumbrennen, K.B., (2008) Biochim. Biophys. Acta 1783 (2), 246-252. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human IREB2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| IREB2 antibody can be used for detection of IREB2 by ELISA at 1:312500. IREB2 antibody can be used for detection of IREB2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IREB2 |
|---|---|
| Alternative Names | IREB2 ACO3 FLJ23381 IRP2 IRP2AD |
| Molecular Weight(MW) | 105 kDa |
Application
Antibody used in WB on Human Liver at 0.2-1 ug/ml.
Application
Antibody used in WB on Mouse Liver at: 1:50.| Positive Control | 1) Cat. No. XBL-10409 - Fetal Liver Tissue Lysate |
|---|---|
| Application Notes | IREB2 antibody can be used for detection of IREB2 by ELISA at 1:312500. IREB2 antibody can be used for detection of IREB2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store IREB2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
