Application
Immunocytochemistry of IRF4 in Jurkat cells with IRF4 antibody at 10 μg/mL.Application
Immunofluorescence of IRF4 in Jurkat cells with IRF4 antibody at 20 μg/mL.Application
Western blot analysis of IRF4 in Ramos cell lysate with IRF4 antibody at 0.5 μg/mL.| Product Name | IRF4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | IRF4 Antibody: Interferons (IFNs) are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. Another member, IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. IRF4 expression is tightly regulated in resting primary T cells and plays an essential role in the homeostasis and function of mature lymphocytes. IRF4 is induced by Toll-like receptor (TLR) activation and acts as a negative regulator of TLR signaling.1) Malmgaard L. Induction and regulation of IFNs during viral infections. J. Interferon & Cyto. Res. 2004; 24:439-54 |
| Immunogen | IRF4 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human IRF4. The immunogen is located within the last 50 amino acids of IRF4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| IRF4 antibody can be used for detection of IRF4 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IRF4 |
|---|---|
| Alternative Names | IRF4 Antibody: MUM1 LSIRF NF-EM5 MUM1 Interferon regulatory factor 4 Lymphocyte-specific interferon regulatory factor IRF-4 |
| Sequence Similarities | 2) Cat. No. 17-005 - Jurkat Cell Slide |
| Tissue Specificity | IRF4 antibody is predicted to not cross-react with other IRF protein family members. IRF4 often migrates at a higher than predicted molecular weight in SDS-PAGE. |
Application
Immunocytochemistry of IRF4 in Jurkat cells with IRF4 antibody at 10 μg/mL.
Application
Immunofluorescence of IRF4 in Jurkat cells with IRF4 antibody at 20 μg/mL.
Application
Western blot analysis of IRF4 in Ramos cell lysate with IRF4 antibody at 0.5 μg/mL.| Positive Control | 1) Cat. No. 1225 - Ramos Cell Lysate |
|---|---|
| Application Notes | IRF4 antibody can be used for detection of IRF4 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | IRF4 antibody can be stored at 4 ˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IRF4 Antibody is supplied in PBS containing 0.02% sodium azide. |
