Application
Antibody used in IHC on Human Skin.Application
Antibody used in WB on Human Jurkat 5.0 ug/ml.| Product Name | IRF4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | IFN regulatory factor (IRF)-4 is a lymphoid/myeloid-restricted member of the IRF transcription factor family that plays an essential role in the homeostasis and function of mature lymphocytes. IRF-4 expression is tightly regulated in resting primary T cells and is transiently induced at the mRNA and protein levels after activation by Ag-mimetic stimuli such as TCR cross-linking or treatment with phorbol ester and calcium ionophore (PMA/ionomycin).1) Lehtonen, A., (2005) J. Immunol. 175 (10), 6570-6579. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human IRF4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| IRF4 antibody can be used for detection of IRF4 by ELISA at 1:312500. IRF4 antibody can be used for detection of IRF4 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IRF4 |
|---|---|
| Alternative Names | IRF4 LSIRF MUM1 NF-EM5 |
| Molecular Weight(MW) | 52 kDa |
Application
Antibody used in IHC on Human Skin.
Application
Antibody used in WB on Human Jurkat 5.0 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | IRF4 antibody can be used for detection of IRF4 by ELISA at 1:312500. IRF4 antibody can be used for detection of IRF4 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store IRF4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
