Application
Immunohistochemistry of IRGM in human brain tissue with IRGM antibody at 5 μg/mL.Application
Immunofluorescence of IRGM in Human Brain tissue with IRGM antibody at 20 μg/mL.Application
Western blot analysis of IRGM in human brain lysate with IRGM antibody at (A) 1 and (B) 2 μg/mL.| Product Name | IRGM Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | IRGM Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Two of the strongest hits implicate genes IRGM and ATG16L1, which encode proteins thought to be critical to the autophagy pathway and being significantly associated with Crohn's disease. IRGM induces autophagy and generates large autolysosomal organelles as a mechanism for the elimination of intracellular Mycobacterium tuberculosis. In mouse, IRGM belongs to a family of gamma-interferon-induced GTP-binding proteins of approximately 48 kDa that also includes IRGM2 and IRGM3; this antibody may also recognize these proteins.1) Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene 2004; 23:2891-906. |
| Immunogen | IRGM antibody was raised against a 14 amino acid synthetic peptide near the center of the human IRGM. The immunogen is located within the last 50 amino acids of IRGM. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| IRGM antibody can be used for detection of IRGM by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IRGM |
|---|---|
| Alternative Names | IRGM Antibody: IFI1 IRGM1 LRG47 LRG-47 IFI1 Immunity-related GTPase family M protein Immunity-related GTPase family M protein 1 |
| Molecular Weight(MW) | Predicted: 20 kDa Observed: 24 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | IRGM antibody may recognize mouse IRGM2 and IRGM3. |
Application
Immunohistochemistry of IRGM in human brain tissue with IRGM antibody at 5 μg/mL.
Application
Immunofluorescence of IRGM in Human Brain tissue with IRGM antibody at 20 μg/mL.
Application
Western blot analysis of IRGM in human brain lysate with IRGM antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | IRGM antibody can be used for detection of IRGM by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | IRGM antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IRGM Antibody is supplied in PBS containing 0.02% sodium azide. |
