ISGF3G functions to recruit RNA polymerase II to the promoter of interferon-stimulated genes and requires histone deacetylases. Defects in ISGF3 can cause resistance to IFN-. (2a) treatment.1) Laver, T., (2008) J. Interferon Cytokine Res. 28 (1), 13-23.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ISGF3G.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ISGF3G antibody can be used for detection of ISGF3G by ELISA at 1:62500. ISGF3G antibody can be used for detection of ISGF3G by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Liver.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
ISGF3G antibody can be used for detection of ISGF3G by ELISA at 1:62500. ISGF3G antibody can be used for detection of ISGF3G by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ISGF3G antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.