ISL2 is the transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways.1) Li, Y., (2007) Mol. Biol. Rep. 34 (1), 19-26.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ISL2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ISL2 antibody can be used for detection of ISL2 by ELISA at 1:62500. ISL2 antibody can be used for detection of ISL2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.
Positive Control
1) Tranfected 293T Cell Lysate
Application Notes
ISL2 antibody can be used for detection of ISL2 by ELISA at 1:62500. ISL2 antibody can be used for detection of ISL2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ISL2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.