Isl2 specifies RGC laterality by repressing an ipsilateral pathfinding program unique to VTC RGCs and involving Zic2 and EphB1. This genetic hierarchy controls binocular vision.1) Svard, J., et al., (2006) Dev. Cell 10 (2), 187-197.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ISL2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ISL2 antibody can be used for detection of ISL2 by ELISA at 1:312500. ISL2 antibody can be used for detection of ISL2 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
ISL2 antibody can be used for detection of ISL2 by ELISA at 1:312500. ISL2 antibody can be used for detection of ISL2 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ISL2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt