Myoinositol, the most common naturally occurring form of inositol, is a component of plasma membrane phospholipids and functions as a cell signaling molecule. ISYNA1 (EC 5.5.1.4), or IPS, is a rate-limiting enzyme that catalyzes the de novo synthesis of myoinositol 1-phosphate from glucose 6-phosphate.Myoinositol, the most common naturally occurring form of inositol, is a component of plasma membrane phospholipids and functions as a cell signaling molecule. ISYNA1 (EC 5.5.1.4), or IPS, is a rate-limiting enzyme that catalyzes the de novo synthesis of myoinositol 1-phosphate from glucose 6-phosphate (Seelan et al., 2004 [PubMed 15464731]).1) Seelan, R.S., (2004) Arch. Biochem. Biophys. 431 (1), 95-106.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ISYNA1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ISYNA1 antibody can be used for detection of ISYNA1 by ELISA at 1:62500. ISYNA1 antibody can be used for detection of ISYNA1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Hum. Adult Placenta at 1 ug/ml.
Application
Antibody used in WB on Human Lung at 0.2-1 ug/ml.
Positive Control
1) Cat. No. XBL-10410 - Fetal Lung Tissue Lysate
Application Notes
ISYNA1 antibody can be used for detection of ISYNA1 by ELISA at 1:62500. ISYNA1 antibody can be used for detection of ISYNA1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ISYNA1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.