Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using ITCH (Phospho-Tyr420) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | ITCH (phospho Tyr420) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain.1) Chen X., Genomics 73:238-241(2001). |
| Immunogen | ITCH (phospho Tyr420) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 420 (F-I-Y (p)-G-N) derived from Human ITCH . |
| Modification | p-Tyr420 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ITCH |
|---|---|
| Alternative Names | AIF4 AIP4 NAPP1 |
| Molecular Weight(MW) | 102 kDa |
| Tissue Specificity | The antibody detects endogenous levels of ITCH only when phosphorylated at tyrosine 420. |
| Entrez Gene | 83737 |
|---|---|
| Protein Accession | NP_001244066.1 |
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using ITCH (Phospho-Tyr420) antibody (left) or the same antibody preincubated with blocking peptide (right).| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
