Application
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.| Product Name | ITGB3BP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ITGB3BP, CENP-R, CENPR, HSU37139, NRIF3, TAP20 |
| Product description | ITGB3BP is a transcription coregulator that can have both coactivator and corepressor functions. Isoform 1, but not other isoforms, is involved in the coactivation of nuclear receptors for retinoid X (RXRs) and thyroid hormone (TRs) in a ligand-dependent fashion. ITGB3BP acts as a transcriptional corepressor via its interaction with the NFKB1 NF-kappa-B subunit, possibly by interfering with the transactivation domain of NFKB1. It induces apoptosis in breast cancer cells, but not in other cancer cells, via a caspase-2 mediated pathway that involves mitochondrial membrane permeabilization but does not require other caspases. ITGB3BP may also act as an inhibitor of cyclin A-associated kinase. ITGB3BP may be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex.1) Gregory, S.G., (2006) Nature 441 (7091), 315-321. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ITGB3BP. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ITGB3BP antibody can be used for detection of ITGB3BP by ELISA at 1:62500. ITGB3BP antibody can be used for detection of ITGB3BP by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ITGB3BP |
|---|---|
| Alternative Names | ITGB3BP CENP-R CENPR HSU37139 NRIF3 TAP20 |
| Molecular Weight(MW) | 20 kDa |
Application
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | ITGB3BP antibody can be used for detection of ITGB3BP by ELISA at 1:62500. ITGB3BP antibody can be used for detection of ITGB3BP by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ITGB3BP antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
