Application
Western Blot at 1:2000 dilution + human plasma lysate Lysates/proteins at 20 ug per lane.Application
Overlay histogram showing U-2OS cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit I| Product Name | ITIH4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Inter-alpha-trypsin inhibitor heavy chain H4, ITI heavy chain H4, ITI-HC4, Inter-alpha-inhibitor heavy chain 4, Inter-alpha-trypsin inhibitor family heavy chain-related protein, IHRP, Plasma kallikrein sensitive glycoprotein 120, Gp120, PK-120, 70 kDa inte |
| Product description | Type II acute-phase protein (APP) involved in inflammatory responses to trauma. May also play a role in liver development or regeneration.1) Nishimura H.,et al.FEBS Lett. 357:207-211(1995). |
| Immunogen | This ITIH4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 784-816 amino acids from human ITIH4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For FACS starting dilution is: 1:25 For WB starting dilution is: 1:2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ITIH4 |
|---|---|
| Alternative Names | Inter-alpha-trypsin inhibitor heavy chain H4 ITI heavy chain H4 ITI-HC4 Inter-alpha-inhibitor heavy chain 4 Inter-alpha-trypsin inhibitor family heavy chain-related protein IHRP Plasma kallikrein sensitive glycoprotein 120 Gp120 PK-120 70 kDa inte |
| Molecular Weight(MW) | 103 kDa |
Application
Western Blot at 1:2000 dilution + human plasma lysate Lysates/proteins at 20 ug per lane.
Application
Overlay histogram showing U-2OS cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit I| Application Notes | For FACS starting dilution is: 1:25 For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
