Application
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of ITM2A using rabbit polyclonal ITM2A Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ITM2A gene (Lane 2).Application
Western blot analysis of ITM2A Antibody in K562 cell line lysates (35ug/lane)| Product Name | ITM2A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Integral membrane protein 2A, Protein E25, ITM2A |
| Product description | ITM2A is induced during thymocyte selection and T cell activation and causes downregulation of CD8 when overexpressed in CD4(+)CD8(+) double positive thymocytes.1) Gudbjartsson,D.F., et.al., Nat. Genet. 40 (5), 609-615 (2008) |
| Immunogen | This ITM2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-49 amino acids from the N-terminal region of human ITM2A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | ITM2A |
|---|---|
| Alternative Names | Integral membrane protein 2A Protein E25 ITM2A |
| Molecular Weight(MW) | 30 kDa |
Application
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of ITM2A using rabbit polyclonal ITM2A Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ITM2A gene (Lane 2).
Application
Western blot analysis of ITM2A Antibody in K562 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
