Application
Western blot analysis in mouse cerebellum,lung tissue lysates (35ug/lane).Application
ITM2B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | ITM2B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Amyloid precursor proteins are processed by beta-secretase and gamma-secretase to produce beta-amyloid peptides which form the characteristic plaques of Alzheimer disease. This gene encodes a transmembrane protein which is processed at the C-terminus by furin or furin-like proteases to produce a small secreted peptide which inhibits the deposition of beta-amyloid. Mutations which result in extension of the C-terminal end of the encoded protein, thereby increasing the size of the secreted peptide, are associated with two neurogenerative diseases, familial British dementia and familial Danish dementia.1) Peng, S., et al. Biochem. Biophys. Res. Commun. 393(3):356-361(2010) |
| Immunogen | This ITM2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-247 amino acids from the C-terminal region of human ITM2B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For IHC-P starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 | |
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ITM2B |
|---|---|
| Alternative Names | Integral membrane protein 2B Immature BRI2 imBRI2 Protein E25B Transmembrane protein BRI Bri BRI2 membrane form Mature BRI2 mBRI2 BRI2 intracellular domain BRI2 ICD BRI2C soluble form Bri23 peptide Bri2-23 ABri23 C-terminal peptide P23 pe |
| Molecular Weight(MW) | 30 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Western blot analysis in mouse cerebellum,lung tissue lysates (35ug/lane).
Application
ITM2B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For IHC-P starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
