Application
Western blot analysis of hITPKA-E360 in K562 cell line and mouse lung tissue lysates (35ug/lane)Application
Western Blot at 1:1000 dilution Lane 1: human brain lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | ITPKA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Inositol-trisphosphate 3-kinase A, Inositol 1,5-trisphosphate 3-kinase A, IP3 3-kinase A, IP3K A, InsP 3-kinase A, ITPKA |
| Product description | ITPKA regulates inositol phosphate metabolism by phosphorylation of second messenger inositol 1,4,5-trisphosphate to Ins(1,3,4,5)P4. The activity of the inositol 1,4,5-trisphosphate 3-kinase is responsible for regulating the levels of a large number of inositol polyphosphates that are important in cellular signaling. Both calcium/calmodulin and protein phosphorylation mechanisms control its activity. It is also a substrate for the cyclic AMP-dependent protein kinase, calcium/calmodulin- dependent protein kinase II, and protein kinase C in vitro. ITPKA and ITPKB are 68% identical in the C-terminus region.1) Gonzalez, B., et al., Mol. Cell 15(5):689-701 (2004). |
| Immunogen | This ITPKA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 345-375 amino acids from the C-terminal region of human ITPKA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | ITPKA |
|---|---|
| Alternative Names | Inositol-trisphosphate 3-kinase A Inositol 1 5-trisphosphate 3-kinase A IP3 3-kinase A IP3K A InsP 3-kinase A ITPKA |
| Molecular Weight(MW) | 51 kDa |
Application
Western blot analysis of hITPKA-E360 in K562 cell line and mouse lung tissue lysates (35ug/lane)
Application
Western Blot at 1:1000 dilution Lane 1: human brain lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
